Recombinant Rat Cardiotrophin-1 (rRtCT-1)

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英文全名

Recombinant Rat Cardiotrophin-1 (rRtCT-1)

英文别名

蛋白结构

Squence of Recombinant Rat Cardiotrophin-1

氨基酸序列

MSQREGSLED HQTDSSFSFL PHLEAKIRQT HNLARLLTKY ADQLLEEYVQ QQGEPFGLPG FSPPRLPLAG LSGPAPSHAG LPVSERLRQD AAALSALPAL LDAVRRRQAE LNPRAPRLLR SLEDAARQVR ALGAAVETVL AALGAAARGP VPEPVATSAL FTSNSAAGVF SAKVLGLHVC GLYGEWVSRT EGDLGQLVPG GVA

Accession

Q63086

Gene ID

29201

产品英文概述

Cardiotrophin­1 (CT­1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung. Studies showed CT-1 which induces cardiac myocyte hypertrophy in vitro can bind to and activate the ILST/gp130 receptor. Rat CT­1 encodes a 203 amino acid (a.a.) residue protein that lacks a hydrophobic signal peptide and it shares 94 % a.a. and 79 % a.a. sequence identity with human and murine CT-1.

来源

Escherichia coli.

分子量

Approximately 21.4 kDa, a single non-glycosylated polypeptide chain containing 203 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile, 0.1 % TFA.

内毒素

Less than 1 EU/ug of rRtCT-1 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4mM HCl to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

SDS-PAGE of Recombinant Rat Cardiotrophin-1

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Rat Cardiotrophin-1是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Rat Cardiotrophin-1 (rRtCT-1)