Recombinant Human Trefoil Factor 2 (rHuTFF2)
英文全名
Recombinant Human Trefoil Factor 2 (rHuTFF2)
英文别名
Spasmolysin, Spasmolytic polypeptide, SP
蛋白结构
氨基酸序列
EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY
Accession
Gene ID
产品英文概述
来源
Escherichia coli.
分子量
Approximately 12.0 kDa, a single non-glycosylated polypeptide chain containing 106 amino acids, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
生物活性
Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
外观
Sterile filtered white lyophilized (freeze-dried) powder.
配方
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
内毒素
Less than 1 EU/ug of rHuTFF2 as determined by LAL method.
溶解说明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
参考资料
1. Seib T, Blin N, Hilgert K, et al. 1997. Genomics, 40: 200-2.2. Kirikoshi HandKatoh M. 2002. Int J Oncol, 21: 655-9.3. Xue L, Aihara E, Podolsky DK, et al. 2010. Gut, 59: 1184-91.4. Poulsen SS, Thulesen J, Christensen L, et al. 1999. Gut, 45: 516-22.
纯度
> 97 % by SDS-PAGE and HPLC analyses.
SDS-PAGE电泳
包装规格
5ug/100ug/500ug
安全数据表下载 (SDS)
技术参数表下载 (TDS)
品牌链接
Recombinant Human Trefoil Factor 2是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Trefoil Factor 2 (rHuTFF2)
