SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein, Accession: QHD43416

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  • 产品概述
  • 产品规格
  • 运输及存储
  • 品牌声明

    • 产品概述

    产品描述

    Recombinant SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer Protein is expressed from HEK293 with His tag at the C-terminal.It contains Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

    Biotinylated

    产品规格

    靶点

    Spike S (B.1.640.2/IHU) Trimer

    分子别名

    Accession

    QHD43416

    种属

    SARS-COV-2

    蛋白序列

    Val16-Pro1213(E96Q, CNDPFLGV136-144del, R190S, D215H, R346S, N394S, Y449N, E484K, F490S, N501Y, D614G, P681H, T859N, D1139H). Proline substitutions(F817P, A892P, A899P, A942P, K986P, V987P)and alanine substitutions(R683A and R685A)are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

    分子量

    The protein has a predicted MW of 133.60 kDa. Due to glycosylation, the protein migrates to 140-160 kDa based on Tris-Bis PAGE result.

    活性

    Immobilized SARS-COV-2 Spike S (B.1.640.2/IHU) Trimer, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 89.9ng/ml determined by ELISA. See testing image for detail.

    产品标签

    C-His

    表达系统

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产品外观

    Lyophilized

    运输条件

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和稳定性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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