Human GP100 (HLA-A*02:01) Tetramer Protein, Accession: A0A140T913

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  • 产品概述
  • 产品规格
  • 运输及存储
  • 品牌声明

    • 产品概述

    产品描述

    Recombinant Human GP100 (HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide.

    Biotinylated

    产品规格

    靶点

    GP100 (HLA-A*02:01) Tetramer

    分子别名

    HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100

    Accession

    A0A140T913

    种属

    Human

    蛋白序列

    Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide

    分子量

    The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

    活性

    Immobilized Human GP100 (HLA-A*02:01) Tetramer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GP100 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. The affinity constant of 0.196 nM as determined in SPR assay (Biacore T200). See testing image for detail.

    产品标签

    C-His-Avi

    表达系统

    HEK293

    纯度

    > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

    内毒素

    Less than 1EU per ug by the LAL method.

    运输及存储

    产品外观

    Lyophilized

    运输条件

    Shipped at ambient temperature.

    制剂

    Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

    复溶

    Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

    存储和稳定性

    Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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