Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor (rHuMANF)
英文全名
Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor (rHuMANF)
英文别名
ARMET
蛋白结构
氨基酸序列
LRPGDCEVCI SYLGRFYQDL KDRDVTFSPA TIENELIKFC REARGKENRL CYYIGATDDA ATKIINEVSK PLAHHIPVEK ICEKLKKKDS QICELKYDKQ IDLSTVDLKK LRVKELKKIL DDWGETCKGC AEKSDYIRKI NELMPKYAPK AASARTDL
Accession
Gene ID
产品英文概述
MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF, each contain an N-terminal saposin-like lipid binding domain, and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson disease. Mature human MANF is 99 %, 98 % and 96 % a.a. identical to mature rat, mouse and bovine MANF respectively.
来源
Escherichia coli.
分子量
Approximately 18.2 kDa, a single non-glycosylated polypeptide chain containing158 amino acids.
生物活性
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 20 ug/ml, corresponding to a specific activity of > 50 IU/mg.
外观
Sterile filtered white lyophilized (freeze-dried) powder.
配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
内毒素
Less than 1 EU/ug of rHuMANF as determined by LAL method.
溶解说明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
参考资料
纯度
> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE电泳
包装规格
5ug/100ug/500ug
安全数据表下载 (SDS)
技术参数表下载 (TDS)
品牌链接
Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor (rHuMANF)
