Recombinant Human Retinoblastoma-Associated Protein Fragment, 137a.a. with His-tag (rHuRb137,His)

英文全名

英文别名

p105-Rb, pRb, pp110

蛋白结构

氨基酸序列

MASFPSSPLR IPGGNIYISP LKSPYKISEG LPTPTKMTPR SRILVSIGES FGTSEKFQKI NQMVCNSDRV LKRSAEGSNP PKPLKKLRFD IEGSDEADGS KHLPGESKFQ QKLAEMTSTR TRMQKQKMND SMDTSNKEEK HHHHHH

Accession

Gene ID

产品英文概述

Rb encoded by the RB1 gene in humans, is expressed by retina and belongs to the etinoblastoma-associated protein family. The hole protein consists of 928 a.a. and the rHuRb fragment occupies sequence of 792-929 a.a.. Rb is a key regulator of entry into cell division that acts as a tumor suppressor. It has many functions, for example, promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C, and acts as a transcription repressor of E2F1 target genes and so on. The rHuRb is the region that rich of modified residue like phosphothreonine and N6-acetyllysine.

来源

Escherichia coli.

分子量

Approximately 16.5 kDa, a single non-glycosylated polypeptide chain containing 146 amino acids.

生物活性

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rHuRb137, His as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Lee WH, Shew JY, Hong FD, et al. 1987. Nature, 329: 642-5.2. Lee WH, Bookstein R, Hong F, et al. 1987. Science, 235: 1394-9.3. Toguchida J, McGee TL, Paterson JC, et al. 1993. Genomics, 17: 535-43.4. Fattaey AR, Helin K, Dembski MS, et al. 1993. Oncogene, 8: 3149-56.

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/100ug/500ug

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human Retinoblastoma-Associated Protein Fragment, 137a.a. with His-tag是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Retinoblastoma-Associated Protein Fragment, 137a.a. with His-tag (rHuRb137,His)