Recombinant Murine Macrophage Colony Stimulating Factor (rMuM-CSF)

英文全名

英文别名

CSF-1, MGI-IM

蛋白结构

氨基酸序列

KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE

Accession

Q3U4F9

Gene ID

产品英文概述

来源

Escherichia coli.

分子量

Approximately 52.0 kDa, a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4.

内毒素

Less than 1 EU/ug of rMuM-CSF as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26.2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52.3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35.4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102.

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/100ug/500ug

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品牌链接

Recombinant Murine Macrophage Colony Stimulating Factor是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Murine Macrophage Colony Stimulating Factor (rMuM-CSF)