Recombinant Tobacco Etch Virus Protease S219V, GST tagged (rTEVS219V,GST)

英文全名

英文别名

蛋白结构

氨基酸序列

Accession

Gene ID

产品英文概述

TEV protease encoded by the tobacco etch virus is a catalytic domain of the Nuclear Inclusion a (NIa) protein. It consists of 241 aa with the molecular weight of 27 kDa. TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X’, and cleaves between Q (or S)/X’. In this form X and X’ stand for any of the amino acid residues, except that X’ cannot be P. The optimal cleavage site is ENLYFQ/G. However, a serious drawback of TEV protease is that it readily cleaves itself at a specific site to generate a truncated enzyme with greatly diminished activity. The mutants, S219V, was not only far more stable than the wild-type protease (~100-fold), but also a more efficient catalyst. As having the absolute specificity and wildly using conditions like broad pH range and ionic strength, the TEV protease became more versatile than EK, thrombin and other protease used in biochemical applications, especially recombinant protein production. The optimal temperature for cleavage is 30 °C; however, the enzyme can be used at temperatures as low as 4 °C. Following digestion, TEV Protease can be removed from the reaction via the GST tag sequence by GST chromatography.

来源

Escherichia coli.

分子量

生物活性

外观

配方

内毒素

溶解说明

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.

参考资料

纯度

A 0.2 um filtered solution in 25mM Tris-HCl, pH 8.0, 0.25 mM EDTA, 0.5Mm DTT, with 50% Glycerol.

SDS-PAGE电泳

包装规格

300IU/1kIU

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品牌链接

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