Recombinant Human Interleukin-12 (rHuIL-12)
英文全名
Recombinant Human Interleukin-12 (rHuIL-12)
英文别名
Natural killer cell stimulatory factor chain 2, NKSF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40
蛋白结构
氨基酸序列
HuIL-12 p40: Ile23 – Ser328; Accession # P29460HuIL-12 p35: Arg23 – Ser219; Accession # P29459
Accession
Gene ID
产品英文概述
IL-12 is a heterodimeric cytokine of disulfide-linked p35 and p40 subunits. It is secreted by monocytes, macrophages, dendritic cells, neutrophils, Langerhans cells, keratinocytes, microglia, and peripheral B cells. In humans, the p40 subunit can also associate with a p19 subunit to form IL-23. IL-12 signals through a receptor complex consisting of IL-12 R beta 1 and IL-12 R beta 2. It promotes Th1 immune responses by inducing IFN-gamma secretion from NK cells, T cells, and macrophages and by enhancing NK cell and T cell mediated cytotoxicity. It also cooperates with IL-18 in the generation of Th1 memory cells.
来源
Spodoptera frugiperda, Sf21 (baculovirus)
分子量
Apparent molecular mass of 60 kDa in SDS-PAGE under non-reducing conditions, 41 kDa and 29 kDa under reducing conditions, a disulfide-linked heterodimer consisting of the 306 amino acid rHuIL-12 p40 and the 197 amino acid rHuIL-12 p35 subunits.
生物活性
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rHuIL-12 is approximately 1.1 × 104 units/ug, which is calibrated against rHuIL-12 WHO Standard (NIBSC code: 95/544).
外观
Sterile filtered white lyophilized (freeze-dried) powder.
配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.2.
内毒素
Less than 1.0 EU/ug of rHuIL-12 as determined by LAL method.
溶解说明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriately buffered solutions.
储存条件
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
参考资料
纯度
> 97 % by SDS-PAGE.
SDS-PAGE电泳
包装规格
10ug/100ug/500ug
安全数据表下载 (SDS)
技术参数表下载 (TDS)
品牌链接
Recombinant Human Interleukin-12是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Interleukin-12 (rHuIL-12)
