Recombinant Human Interleukin-15 GMP (rHuIL-15) - 江苏沃尔森生物科技有限公司

英文全名

Recombinant Human Interleukin-15 GMP (rHuIL-15)

英文别名

IL-T

蛋白结构

氨基酸序列

NWVNVISDLK KIEDLIQSMH IDATLYTESD VHPSCKVTAM KCFLLELQVI SLESGDASIH DTVENLIILA NNSLSSNGNV TESGCKECEE LEEKNIKEFL QSFVHIVQMF INTS

Accession

P40933

Gene ID

3600

产品英文概述

Human Interleukin-15 (IL-15) is expressed by the IL15 gene located on the chromosome 4. It shares approximately 97 % and 73 % sequence identity with simian and murine IL-15, respectively. Both human and simian IL-15 are active on murine cells. IL-15 is secreted by mononuclear phagocytes (and some other cells), especially macrophages following infection by virus. It possesses a variety of biological functions, including stimulating and maintaining of cellular immune responses, especially regulating T and natural killer (NK) cell activation and proliferation. In additionally, it shares many biological properties with IL-2, including T, B and NK cell-stimulatory activities. IL-15 signals through a complex composed of IL-2/IL-15 receptor beta chain. Although IL-15 lacks sequence homology with IL-2, it has recently been shown that both the beta and gamma chains of the IL-2 receptor are utilized for IL-15 binding and signaling. In addition, an IL-15 specific binding protein has also been cloned from a mouse T cell clone.

来源

Escherichia coli.

分子量

Approximately 12.9 kDa, a single non-glycosylated polypeptide chain containing 114 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 0.01 EU/ug of rHuIL-15 GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Anderson DM, Johnson L, Glaccum MB, et al. 1995. Genomics, 25: 701-6.2. Krause H, Jandrig B, Wernicke C, et al. 1996. Cytokine, 8: 667-74.3. Chirifu M, Hayashi C, Nakamura T, et al. 2007. Nat Immunol, 8: 1001-7.4. Grabstein KH, Eisenman J, Shanebeck K, et al. 1994. Science, 264: 965-8.5. Giri JG, Ahdieh M, Eisenman J, et al. 1994. EMBO J, 13: 2822-30.6. Arena A, Merendino RA, Bonina L, et al. 2000. New Microbiol, 23: 105-12.