Recombinant Murine Macrophage Inflammatory Protein-1 beta/CCL4 (rMuMIP-1beta/CCL4)

英文全名

英文别名

ACT-2, MIP-1-beta, Protein H400, SIS-gamma, Small-inducible cytokine A4

蛋白结构

氨基酸序列

APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN

Accession

P14097

Gene ID

20303

产品英文概述

Chemokine (C-C motif) ligand 4, also known as Macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant murine CCL4 contains 69 amino acids and it shares 77 % and 86 % a.a. sequence identity with human and rat CCL4. Both human and murine MIP-1alpha and MIP-1β are active on human and murine hematopoietic cells.

来源

Escherichia coli.

分子量

Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.

生物活性

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 20-100 ng/ml.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4.

内毒素

Less than 1 EU/ug of rMuMIP-1β/CCL4 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 97 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/100ug/500ug

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品牌链接

Recombinant Murine Macrophage Inflammatory Protein-1 beta/CCL4是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Murine Macrophage Inflammatory Protein-1 beta/CCL4 (rMuMIP-1beta/CCL4)